Publications

Nature Versus Nurture: Defining the Effects of Mesenchymal Stromal Cell Isolation and Culture Conditions on Resiliency to Palmitate Challenge.
Boland, L., Burand, A., Boyt, D.,  Dobroski, H., Di, L., Liszewski, J., Schrodt, M., Frazer, M., Santillan, D., Ankrum, J. (2019) Frontiers in Immunology.  doi:10.3389/fimmu.2019.01080

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates.
Liu, S., Harata, M., Promes, J., Burand, A ., Ankrum, J ., Imai, Y. (2019) Journal of Visualized Experiments: JoVE, Issue 147. doi:10.3791/59578

Hepatic lysosomal iNOS activity impairs autophagy in obesity.
Qian, Q., Zhang, Z., Li, M., Savage, K., Cheng, D., Rauckhorst, A., Ankrum, J. , Taylor, E., Ding, W., Xiao, Y., Cao, H., Yang, L. (2019). Cellular and Molecular Gastroenterology and Hepatology. Vol. 8(1), 95-110

Intravascular mesenchymal stromal/stem cell therapy product diversification: time for new clinical guidelines.
Moll, G. Ankrum, J ., Kamhieh-Milz, J., Bieback, K., Ringd.n, O., Volk, H. Geissler, S., Reinke, P. (2019).  Trends in molecular medicine. Vol.25(2), p.149-163.

Manufacturing of primed mesenchymal stromal cells for therapy.
Yin, J., Zhu, J., Ankrum, J . (2019)  Nature Biomedical Engineering. 3, 90-104

Delivery of shRNA via lentivirus in human pseudoislets provides a model to test dynamic regulation of insulin secretion and gene function in human islets.
Harata, M., Liu, S., Promes, J. A., Burand, A. J., Ankrum, J., & Imai, Y. (2018) Physiological Reports, 6(20), e13907. http://doi.org/10.14814/phy2.13907

From lectures to the lab: three steps to becoming an undergraduate researcher
Ankrum J. Nature 2018 July 30. doi:10.1038/d41586-018-05823-5

Scaffold-free generation of uniform adipose spheroids for metabolism research and drug discovery
Klingelhutz AJ, Gourronc FA, Chaly A, Wadkins DA, Burand AJ, Markan KR, Idiga SO, Wu M, Potthoff MJ, Ankrum JA, Scientific Reports 8, Article number: 523 (2018) doi:10.1038/s41598-017-19024-z

IFN-γ and TNF-α Pre-licensing Protects Mesenchymal Stromal Cells from the Pro-Inflammatory Effects of Palmitate
Boland L, Burand AJ,  Brown AJ, Boyt D, Lira VA, Ankrum J. Molecular Therapy 2017 Dec 14. doi: 10.1016/j.ymthe.2017.12.013

A Low-Cost Technique for Intraoperative Imaging of Cell Delivery and Retention in a Model of Delayed Wound Healing
Burand AJ, Boland L, Brown AJ, Ankrum J. Advances in Wound Care (2017).

Function of Cryopreserved Mesenchymal Stromal Cells With and Without Interferon-γ Prelicensing is Context Dependent.
Burand, A J, Gramlich, OW, Brown, A J & Ankrum, J. Stem Cell (2016). doi:10.1002/stem.2528 

Targeted Strategies to Modulate Stem-Cell-Relevant Pathways.
Lowery, J. W., Ankrum JA, Kokabu S, Liu R. Stem Cells International 2016, 1–2 (2016). 

Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy 
Gramlich OW , Burand AJ , Brown AJ, Deutsch RJ, Kuehn MH, Ankrum JA.
Scientific reports 6, 26463, doi:10.1038/srep26463

Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine. 
Ali MM, Li F, Zhang Z, Zhang K, Kang DK, Ankrum JA, Le XC, Zhao W. Chem Soc Rev. 2014 May 21;43(10):3324-41. doi: 10.1039/c3cs60439j. Epub 2014 Mar 18. PubMed PMID: 24643375.

Performance-enhanced mesenchymal stem cells via intracellular delivery of steroids. 
Ankrum JA, Dastidar RG, Ong JF, Levy O, Karp JM. Sci Rep. 2014 Apr 10;4:4645. doi: 10.1038/srep04645. PubMed PMID: 24717973; PubMed Central PMCID: PMC3982175.

Mesenchymal stem cells: immune evasive, not immune privileged.
Ankrum JA, Ong JF, Karp JM. Nature Biotechnology. 2014 Mar;32(3):252-60. doi: 10.1038/nbt.2816. Epub 2014 Feb 23. PubMed PMID: 24561556.

Engineering cells with intracellular agent-loaded microparticles to control cell phenotype. 
Ankrum JA, Miranda OR, Ng KS, Sarkar D, Xu C, Karp JM. Nature Protocols. 2014 Feb;9(2):233-45. doi: 10.1038/nprot.2014.002. Epub 2014 Jan 9. PubMed PMID: 24407352.

Prodrugs as self-assembled hydrogels: a new paradigm for biomaterials. 
Vemula PK, Wiradharma N, Ankrum JA, Miranda OR, John G, Karp JM. Curr Opin Biotechnol. 2013 Dec;24(6):1174-82. doi: 10.1016/j.copbio.2013.02.006. Epub 2013 Mar 1. Review. PubMed PMID: 23465753.

Microstructured barbs on the North American porcupine quill enable easy tissue penetration and difficult removal.
Cho WK, Ankrum JA, Guo D, Chester SA, Yang SY, Kashyap A, Campbell GA, Wood RJ, Rijal RK, Karnik R, Langer R, Karp JM. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21289-94. doi: 10.1073/pnas.1216441109. PMID: 23236138, PMCID: PMC3535670

Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-α-activated endothelial cells via both leukocyte-like and novel mechanisms.
Teo GS, Ankrum JA, Martinelli R, Boetto SE, Simms K, Sciuto TE, Dvorak AM, Karp JM, Carman CV. Stem Cells. 2012 Nov;30(11):2472-86. doi: 10.1002/stem.1198. PMID: 22887987, PMCID: PMC3479371 

Tracking mesenchymal stem cells with iron oxide nanoparticle loaded poly(lactide-co-glycolide) microparticles. 
Xu C, Miranda-Nieves D, Ankrum JA, Matthiesen ME, Phillips JA, Roes I, Wojtkiewicz GR, Juneja V, Kultima JR, Zhao W, Vemula PK, Lin CP, Nahrendorf M, Karp JM. Nano Lett. 2012 Aug 8;12(8):4131-9. doi: 10.1021/nl301658q. PMID: 22769232

Nanoparticle-based monitoring of cell therapy. 
Xu C, Mu L, Roes I, Miranda-Nieves D, Nahrendorf M, Ankrum JA, Zhao W, Karp JM. Nanotechnology. 2011 Dec 9;22(49):494001. doi: 10.1088/0957-4484/22/49/494001. PMID: 22101191, PMCID: PMC3334527 

Cellular and extracellular programming of cell fate through engineered intracrine-, paracrine-, and endocrine-like mechanisms.
*Sarkar D, *Ankrum JA, Teo GS, Carman CV, Karp JM. Biomaterials. 2011 Apr;32(11):3053-61. doi: 10.1016/j.biomaterials.2010.12.036. PMID: 21262537, PMCID: PMC3043463  *Co-first authors

Nasogastric Tube Design to Reduce Clogging and Simplify Flushing. Journal of Medical Devices.
Ankrum JA, Alison OL, Jose C, Elliot G, Michael R. Journal of Medical Devices. 2011 ; 5(2):027510.

Mesenchymal stem cell therapy: Two steps forward, one step back.
Ankrum JA, Karp JM. Trends Mol Med. 2010 May;16(5):203-9. doi: 10.1016/j.molmed.2010.02.005. PMID: 20335067, PMCID: PMC2881950 

Seated human response to simple and complex impacts: Paraspinal muscle activity. International Journal of Industrial Ergonomics
Xia T, Ankrum J, Spratt K, Wilder D. International Journal of Industrial Ergonomics. (2008) vol. 38 (9-10) pp. 767-774

Three-Dimensional Motion Capture Protocol for Seated Operator in Whole Body Vibration.
Rahmatalla S, Xia T, Contratto M, Kopp G, Wilder D,  Frey Law L, Ankrum JA. International Journal of Industrial Ergonomics, (2008) vol. 38 (5-6) pp. 425-433.

A framework to study human response to whole body vibration. 
Rahmatalla S, Xia T, Ankrum JA, Wilder D, Frey Law L, Abdel-Malek K, Contratto M, Kopp G. SAE Technical Paper. 2007-01-2474

Published Abstracts

Burand, A.,  Di, L.,  Boland, L., Ankrum, J. (2018)
Aggregation of MSCs Leads to a Shift in Secretome Due to Activation of an Ep Receptor-PGE2 Pathway. Molecular Therapy, 26(5), 214-215.

Boland, L.,  Burand, A.,  VanDerZanden, N., Ankrum, J. (2018)
Alterations in PGE2 and IL-6 Production Partially Drive the Pro-Inflammatory Effects of Palmitate Exposed Mesenchymal Stromal Cells. Molecular Therapy, 26(5), 217-218.

Klingelhutz AJ., Gourronc F, Chaly A, Ankrum J  (2017)
Body on-chip and 3D culture: An overview. The​​​​​​​ FASEB Journal , 31 (1 Supplement), 88.1-88.1.

Gramlich, O. W., Zhu, W., Burand, A., Ankrum, J. & Kuehn, M. H. 
Transplantation of cryopreserved MSC rescues retinal ganglion cells following I/R injury. Invest Ophthalmol Vis Sci 57, 4402–4402 (2016)

Burand A, Brown A, Ankrum J
Development of an Injectable Cell-laden Microniche for Mesenchymal Stem Cell Therapy. Tissue Engineering Part A, 21, S275–S275 (2015).

Zasadny FM,  Ankrum JA,  and Abel ED
Lysosomal reacidification via degradation of PLGA nanoparticles in a lipotoxic cardiomyopathy model. 
Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01307

Ankrum JA, Dastidar RG, Ong JF, Levy O, Karp JM. Enhanced Mesenchymal Stem Cell Immunomodulatory Potency through intracellular microparticles. British Journal of Surgery. 2015, v102, pg165

Ong JF, Ankrum JA, Dastidar RG, Levy O, Karp JM. Enhanced mesenchymal stem cell immunomodulatory potency through intracellular budesonide microparticles. British Journal of Surgery. 2014, v101, pg 36

Xia T, Wilder D, Ankrum JA, Spratt K. Electromyographic response of the trunk muscles to complex impacts in seated subjects. Spine Journal Meeting Abstracts. 2007, pg 253.

Wilder D, Xia T, Ankrum JA, Spratt K. Seated human response to simple and complex impacts. First American Conference on Human Vibrations. 2006.

Published Patents

(Due to 18 month delay in publishing, list is not exhaustive)

CELLULAR BARCODE 
JA Ankrum, AJ Burand  
US Patent App. 15/068,106
Published March 11, 2016

CROSS-LINKABLE TISSUE BULKING COMPOSITIONS 
J Ankrum, JD Cezo, SL Kangas 
US Patent App. 14/986,904
Published January 4, 2016

DEEP VEIN THROMBOSIS PROHPYLAXIS 
JA Ankrum, JD Cezo
US Patent 20,160,120,729, published May 5, 2016

FINGER-MOUNTED FILING DEVICE 
JA Ankrum
US Patent 20,150,374,093, published December 31, 2015

Karp JM, Cho WK, Laulicht B, Ankrum JA, Karnik RN, Langer R. Devices and Uses thereof, US Patent App. 13/980,503, published January 18, 2012.

Ankrum JA, Olechowski AL, Roberts MP, Canseco JA, Greenblatt EE. Medical Aspiration Apparatus. US Patent App. 13/983,78, published February 7, 2012.

Thesis

Ankrum JA. A microparticle engineering approach to enhance the potency of mesenchymal stem cells, PhD Thesis, MIT Libraries, 2013.

Ankrum, JA. A study of user interaction with written medicine information.  MPhil Thesis, Cambridge University Engineering Department Library 2008.

Invited Book Chapters

Ankrum JA, Bartosh TJ, Yin X, Brown AJ,  Burand Jr AJ, Boland L. Engineering Organoid Systems to Model Health and Disease In: Emerich DF, Orive G, Editors. Cell Therapy: Current Status and Future Directions, Humana Press; 2017.

Sarkar D, Zhao W, Schaefer S, Ankrum JA, Teo G, Karp JM. Applications of Biomaterials in Functional Tissue Engineering In: Ratner BD, Hoffman AS, Schoen FJ, Lemons JE, Editors. BIOMATERIALS SCIENCE: An Introduction to materials in Medicine Waltham, MA: Academic Press; 2012.

Zhao W, Sarkar D, Ankrum JA, Hall S, Loh W, Teo G, Karp JM. Therapeutic applications of mesenchymal stem/multipotent stromal cells In: Appasani R, Appasani K, Editors. Stem Cells & Regenerative Medicine New York: Humana Press; 2011.

Zhao W, Ankrum JA, Sarkar D, Teo W, Kumar N, Karp JM. Stem Cell Homing to Sites of Injury and Inflammation In: Kong L, Putnam H, Schook R, Editors. Stem Cells Revascularization Therapies Boca Raton, FL: CRC Press; 2011.